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KMID : 0931320170170040208
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대한상부위장관.헬리코박터학회지
2017 Volume.17 No. 4 p.208 ~ p.212
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A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media
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Lee Jung-Hwan
Park Ji-Wan
Park Mi-Ri
Na Yoon-Hee
Cho Soo-Jeong
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Abstract
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Background/Aims: Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori.
Materials and Methods: G27, ATCC #43504 and 60190, and primary cultured strains were used. H. pylori strains were cultured for four days under four culture conditions: chocolate agar, Thayer-Martin (TM) agar containing vancomycin-colistin-nystatin inhibitor (VCNI), Brucella agar, and brain heart infusion (BHI) agar containing 5% horse blood and IsoVitaleX (BBLTM BD, USA). Culture of cells in each medium was repeated fourteen times. The growth of H. pylori was measured by using a spectrophotometer.
Results: TM, Brucella, and BHI agars showed mean absorbance values of 0.099, 0.059, 1.410, and 0.913, respectively. These values were significantly different (P=0.030). After post-adjustment by Bonferroni correction, similar growth was noted for in chocolate, Brucella, and BHI agars; however, TM agar significantly suppressed H. pylori growth compared with Brucella agar (P=0.031).
Conclusions: Chocolate, Brucella, and BHI agars provided effective culture conditions for the growth of H. pylori. TM agar containing VCNI suppressed the growth of H. pylori and other organisms.
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KEYWORD
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Agar, Culture, Helicobacter pylori, Media
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